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CABRI:DSMZ_MUTZ Accession_number ACC 132 Cell_line_name A-10 Brief_description confirmed as rat with IEF of AST, MDH, NP and by species-PCR established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described to display properties characteristic of smooth muscle cells Description rat smooth muscle established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described to display properties characteristic of smooth muscle cells confirmed as rat with IEF of AST, MDH, NP and by species-PCR Depositor obtained from ATCC (CRL 1476), Rockville, Maryland, USA Morphology large, epithelial-like, some fibroblast-like, adherent cells growing in monolayers Culture_conditions 80% Dulbecco's MEM + 20% FBS split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent at 37C with 5-10% CO2 about 1- 2 x 106 cells/80 cm 2 Viruses ELISA: reverse transcriptase positive; PCR: SMRV - Properties Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5-1 x 106 cells/ampoule Doubling_time ca. 70-100 hours Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays Fingerprint unique Hinf I-(gtg) 5 DNA profile Cytogenetics rat karyotype Bibliographic_reference Kimes et al., Exp Cell Res, 98: 55 (1976) [PMID: 943301 ]

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